{"created":"2023-07-25T10:24:18.644677+00:00","id":1221,"links":{},"metadata":{"_buckets":{"deposit":"ca64baa0-cbcc-49d5-b89f-f08fc92a5c00"},"_deposit":{"created_by":4,"id":"1221","owners":[4],"pid":{"revision_id":0,"type":"depid","value":"1221"},"status":"published"},"_oai":{"id":"oai:hirokoku-u.repo.nii.ac.jp:00001221","sets":["16:27:124"]},"author_link":[],"control_number":"1221","item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2023-03-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"30","bibliographicPageStart":"21","bibliographic_titles":[{"bibliographic_title":"HIU健康科学ジャーナル"}]}]},"item_10002_description_19":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_10002_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"近年、口腔内細菌と様々な全身疾患との閧連が注目されてぃる。これまでの研究において、cnm およびpa遺伝子を持つミュータンス菌(cnm/pa+ミュータンス菌)が非アルコール性脂肪肝炎（NASH）の発症に影響を及ぼすことが示唆されており、早期発見が課題となっている。 本研究では遺伝子増幅法の 1つである LOOP-mediated isthermal amplification(LAMP)法を用いて、 cnｍ+/pa+ミュータ ンス菌を短時間かつ特異的に増幅するプライマーの作製および条件を検討した。LAMP用プライマーをcnmは4種類(cnm1, 2、 3、 4)、paは3種(pa1 、2、3）設計し、LAMPによる増幅をした結果、短時聞に増幅したプライマーは cnm2 およびPa1であった。 また、ソブリヌス菌、ジンジバリス菌、カンジダ菌を用いて特異性を見た結果、これらのプライマーはミ ュータンス菌以外の口腔内細菌を増幅しなかった。さらに、健常者の唾液を用いた 検討を行った結果、約4人に 1人が NASH増悪因子としてcnm+pa+ミュータンス菌を保菌している事が明らかとなった。","subitem_description_language":"ja","subitem_description_type":"Abstract"}]},"item_10002_full_name_3":{"attribute_name":"著者（英）","attribute_value_mlt":[{"names":[{"name":"Tomita, Syota","nameLang":"en"}]},{"names":[{"name":"Takahara, Yuka","nameLang":"en"}]},{"names":[{"name":"Suzuki, Tsukika","nameLang":"en"}]},{"names":[{"name":"Nakagaki, Konoka","nameLang":"en"}]},{"names":[{"name":"Kimura, Rumi","nameLang":"en"}]},{"names":[{"name":"Nagamine, Kentaro","nameLang":"en"}]}]},"item_10002_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.60172/0000001221","subitem_identifier_reg_type":"JaLC"}]},"item_10002_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"広島国際大学健康科学部"}]},"item_10002_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"2436-1224","subitem_source_identifier_type":"PISSN"}]},"item_10002_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"富田, 翔太","creatorNameLang":"ja"},{"creatorName":"トミタ, ショウタ","creatorNameLang":"ja-Kana"}]},{"creatorNames":[{"creatorName":"髙原, 優花","creatorNameLang":"ja"},{"creatorName":"タカハラ, ユカ","creatorNameLang":"ja-Kana"}]},{"creatorNames":[{"creatorName":"鈴木, 月花","creatorNameLang":"ja"},{"creatorName":"スズキ, ツキカ","creatorNameLang":"ja-Kana"}]},{"creatorNames":[{"creatorName":"中垣, 好花","creatorNameLang":"ja"},{"creatorName":"ナカガキ, コノカ","creatorNameLang":"ja-Kana"}]},{"creatorNames":[{"creatorName":"木村, 留美","creatorNameLang":"ja"},{"creatorName":"キムラ, ルミ","creatorNameLang":"ja-Kana"}]},{"creatorNames":[{"creatorName":"長嶺, 憲太郎","creatorNameLang":"ja"},{"creatorName":"ナガミネ, ケンタロウ","creatorNameLang":"ja-Kana"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2023-06-19"}],"displaytype":"detail","filename":"jhs02_021.pdf","filesize":[{"value":"2.0 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"jhs02_021.pdf","url":"https://hirokoku-u.repo.nii.ac.jp/record/1221/files/jhs02_021.pdf"},"version_id":"7bf88129-fa3c-4c8e-be6c-32622bcf4a3e"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"LAMP","subitem_subject_scheme":"Other"},{"subitem_subject":"ミュータンス菌","subitem_subject_scheme":"Other"},{"subitem_subject":"う蝕","subitem_subject_scheme":"Other"},{"subitem_subject":"遺伝子診断","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"全身疾患に関わる口腔内ミュータンス菌の検出","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"全身疾患に関わる口腔内ミュータンス菌の検出","subitem_title_language":"ja"},{"subitem_title":"Detection of oral mutans associated with systemic disease","subitem_title_language":"en"}]},"item_type_id":"10002","owner":"4","path":["124"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2023-06-19"},"publish_date":"2023-06-19","publish_status":"0","recid":"1221","relation_version_is_last":true,"title":["全身疾患に関わる口腔内ミュータンス菌の検出"],"weko_creator_id":"4","weko_shared_id":-1},"updated":"2024-03-30T05:21:32.950582+00:00"}